Smurf1 antibody
Web31 Mar 2016 · C, sequential IP assay showing the RASSF1A–Smurf1–RhoA complex formation in HeLa cells. Cell lysates were precipitated with anti-RASSF1A antibody and the resulting complexes were precipitated with anti-Smurf1 antibody. D, characterization of Smurf1-binding region of RASSF1A. E, loss of RhoA-inhibiting activity of RASSF1A by … Web1 Jun 2024 · To suppress the expression of Smurf1, we transfected hPTECs with Smurf1 and Smad7- siRNA (sc-41673 and sc-36508; Santa Cruz Biotechnology, Dallas, TX, USA; sequence listed
Smurf1 antibody
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Web29 Dec 2024 · Smurf1 and RhoA could be detected in the whole cell lysates of all groups, suggesting that the whole cell lysates could be used for subsequent Co‐IP experiments. When Smurf1 antibody was used to pull down the protein complex, RhoA expression was detected in the control group (Smurf1−/RhoA−), while Ub expression was low. WebCatalog Number MK204520. Quantity 1 kit. Type qPCR Template Standards. Target Smurf1. Species Mouse. NCBI Full Gene Name SMAD specific E3 ubiquitin protein ligase 1. NCBI Gene Aliases 4930431E10Rik, mKIAA1625. Primer Type Gene-specific Primers.
WebSMURF1 antibody (ABIN781919). Validated for EIA, IF, IHC (p). Tested in Human, Mouse. Order online. English ... Comparison List Basket Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: [email protected]. Home Antibodies ELISA Kits Primary Antibodies. Monoclonal Antibodies; Polyclonal Antibodies; Recombinant Antibodies ... WebSmurf1, a member of the HECT family of E3 ubiquitin ligases, selectively interacts with BMP pathway Smad effectors, leading to Smad protein …
Web10 Apr 2024 · In Western blots, no crossreactivity with recombinant human SMURF1 is observed. ... Human/Mouse/Rat SMURF2 Antibody Monoclonal Mouse IgG2A Clone # 690638 Catalog Number: MAB6916 Rev. 2/7/2024 Page 1 of 1 ... Web26 Nov 2012 · Precipitates were then resolved by 4–12% SDS-PAGE and immunoblotted with anti-ubiquitin and anti-SMURF1 antibodies, respectively. SMURF1 and Tubulin levels were also shown by immunoblotting in 1% Input. C, 0.5 μg of pcDNA4/TO-SMURF1 cDNA was co-transfected with increasing amount of V5-tagged USP9X construct (0, 1, 2, and 5 μg) into …
Web20 Apr 2024 · Apart from SMURF1/2, another E3 ubiquitin ligase that has been shown to influence the canonical TGF-β pathway is NEDD4-L (NEDD4-Like). Inside the nucleus, the linker region of SMAD2/3 is phosphorylated by CDK8 and CDK9 (cyclin-dependent kinase 8/9), which enhances the transcriptional activity of SMAD2/3 by favoring binding to other …
Webdianova offers a wide range of primary antibodies for diverse immunoassays like IHC (FFPE), ELISA or Western Blotting. Read more! supi 5gWebThis SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1. Dilution : WB~~1:2000 IHC-P~~1:10~50: Format : Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium … supi0doWeb20 Mar 2024 · Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level … sup i4Web11 Jun 2024 · Smurf1 antibody was obtained from Invitrogen (Carlsbad, CA, USA), and ErbB2, ErbB3, Smad1 and c-Jun antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). EGFR antibody was purchased from Abcam (Cambridge, UK). sup i1WebDescription. Anti-SMURF1 Antibody. Background Information. Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase … supia ipdisk co krWeb21 Feb 2024 · Smurf1 silencing restores PTEN expression that ameliorates progression of human glioblastoma and sensitizes tumor cells to mTORC1/C2 inhibitor Torin1 Article Full-text available Nov 2024 Qin Xia... supi 5g 3gppWebSMURF1 antibody (ab57573) used in immunohistochemistry at 2ug/ml on formalin fixed and paraffin embedded human stomach. This image was generated using the ascites version … sup i3