Phospho array
WebAug 2, 2024 · Each Human Phospho-Kinase Antibody Array Kit contains most of the necessary components for a ready-to-run assay without the need for specialized … WebA membrane-based antibody array for the parallel determination of the relative phosphorylation of human receptor tyrosine kinases. Validated for analyte detection in cell lysates. Troubleshooting Guide Key Benefits Detects phosphorylation of 49 human receptor tyrosine kinases simultaneously Requires no specialized equipment Principle of the Assay
Phospho array
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WebAug 26, 2013 · The phospho-array profiling showed that the major increase in kinase activity due to CoMTb stimulation was in p38 mitogen activated protein kinase (MAPK), principally the α and γ subunits. p38 phosphorylation was detected at 15 minutes, with a second peak of activity at 120 minutes. High basal extracellular signal-regulated kinase activity ... WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl …
WebHuman EGFR Phosphorylation Antibody Array - Membrane (17 Targets) Sample type Tissue Extracts, Cell Lysate Assay type Semi-quantitative Species reactivity Reacts with: Human Product overview ab134005 is for … WebNational Center for Biotechnology Information
WebHuman Phospho-Kinase Antibody Array (Membrane, 43 Targets) (Y0225) (AbAr-0225-YC) Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. WebPhospho-specific Antibody Arrays Referenced in Primary Literature. There are over 200 references to phospho-specific, membrane-based antibody arrays in the primary literature. Proteome Profiler™ Phospho-specific Antibody Arrays offered by R&D Systems account for 91% of those referenced.
WebWhat Array format do you want? All Arrays. 96-well standard 96-well Fast. 384-well. OpenArray. What type of primers are you looking for? PCR/Sanger Sequencing Primers) Enter target information. Enter / Upload Multiple Targets. Enter Single Sequence. Select a single species to search by sequence. What chromosome position are you interested in? ...
WebA classical method of directly measuring protein phosphorylation involves the incubation of whole cells with radiolabeled 32 P-orthophosphate, the generation of cellular extracts, separation of proteins by SDS-PAGE, and exposure to film. 2, 5 This labor-intensive method requires many multi-hour incubations and the use of radioisotopes. phone has black screen but worksWebImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). how do you measure lung functionWebJul 7, 2024 · Phospho-protein profiling and Western blotting results showed the expression of NF-κB related phosphorylation sites including NF-κB P65 (Ser536), IκBα, IKKβ, PI3K, and AKT was altered in STC1-overexpressed cervical cancer cells. phone has busy signal instead of dial toneWebHuman MAPK Phosphorylation Antibody Array (Membrane, 17 Targets) (ab211061) Assay Hexokinase Activity Assay Kit (Fluorometric) (ab211103) Primary Anti-GFAP antibody (ab90601) View more associated products Overview Product name Human RTK Phosphorylation Antibody Array -Membrane (71 Targets) Sample type Cell Lysate, Tissue … phone has blue screenWebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material. how do you measure lumensWebOct 16, 2024 · Summary for Proteome Profiler Mouse Phospho-RTK Array Kit Kit Summary A membrane-based antibody array for the parallel determination of the relative levels of mouse receptor tyrosine kinase phosphorylation. Validated for analyte detection in cell lysates. Troubleshooting Guide Key Benefits phone has been disabled connect to itunesWebSep 1, 2024 · Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and Their Use in Precision Pediatric Oncology Authors: Jakub Neradil Masaryk University Michal Kyr... phone has busy signal