Phosphinothricin acetyltransferase gene

Author: xbcb

August undefined, 2024

WebThis gene encodes neomycin phosphotransferase, which confers resistance to antibiotics such as kanamycin, gentamicin A, neomycin, and others when expressed in plants. The hpt gene, also isolated from E. coli, codes for hygromycin transferase and confers resistance … WebStrauch, E.; Wohlleben, W.; Puhler, A.: Cloning of a phosphinothricin Nacetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli. Gene, 63, 65–74 (1988) CrossRef CAS PubMed Google Scholar

Resistance to glufosinate is proportional to …

WebNational Center for Biotechnology Information Web10.1007/BF00201636 Abstract L-Phosphinothricin (L-Pt)-resistant plants were constructed by introducing a modified phosphinothricin-N-acetyl-transferase gene (pat) via … t strap shoes from bandolino

pat: Genes List GM Approval Database - ISAAA.org

WebAbstract. Insertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower … WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of … WebApr 23, 2012 · The phosphinothricin expression cassette consists of the NOS promoter (promoter of the A. tumefaciens nopaline synthase gene), the pat cds (phosphinothricin acetyltransferase gene from Streptomyces viridochromogenes), and the NOS terminator (terminator of the A. tumefaciens nopaline synthase gene). t-strap shoes in large sizes

Biolistic Transformation Forage Information System

2.3.1.183: phosphinothricin acetyltransferase - BRENDA

Phosphinothricin acetyltransferase (EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase. This enzyme catalyses the following chemical reaction acetyl-CoA + phosphinothricin CoA + N-acetylphosphinothricin WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) tiafenacil in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions comprising said … phlegethon reviewWebApr 3, 2024 · Resveratrol-enriched rice (RR) includes the stilbene synthase gene for resveratrol synthesis and the phosphinothricin-N-acetyltransferase gene for glufosinate tolerance.To investigate unintended effects resulting from RR’s genetically modified chemical composition, 56 polar and nonpolar secondary metabolites were analyzed with … t strap shoes manufacturer

"WebPhosphinothricin N-acetyltransferase. Gene. bar. Status. UniProtKB reviewed (Swiss-Prot) Organism. Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) Amino acids. 171. Protein existence. Evidence at protein level. Annotation score. 3/5. " - Phosphinothricin acetyltransferase gene

Phosphinothricin acetyltransferase gene

Phosphinothricin Acetyltransferase - an overview

WebGene: pat. Gene Source: Streptomyces viridochromogenes. Product: phosphinothricin N-acetyltransferase (PAT) enzyme. Function: eliminates herbicidal activity of glufosinate … WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from …

Did you know?

WebMar 18, 2016 · Gene: BASTA selectable marker from Streptomyces hygrosopicus- phosphinothricin acetyltransferase gene to confer BASTA resistance as a selectable marker Promoter: 35S promoter from Cauliflower mosaic caulimovirus – CaMV 35S constitutive promoter to drive expression of GUS reporter gene WebFeb 16, 2016 · Wohlleben, W. et al. Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum. Gene 70 , 25–37 (1988).

WebWohlleben W, Arnold W, Broer I, Hillemann D, Strauch E, Pühler A (1988) Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes TÜ494 and its expression in Nicotiana tabacum. Gene … WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis.

WebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ... WebNov 27, 2024 · Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also …

WebApr 1, 2024 · Only few glufosinate-tolerant genes, such as phosphinothricin acetyltransferase ( PAT) and bialaphos resistance ( bar) identified from Streptomyces, are currently available for developing genetically modified rice in agricultural application.

WebMar 9, 2024 · Plasmid pGL2, bearing the hygromycin phosphotransferase gene ( hph) coding sequence under control of the CaMV35S promoter, is used for hygromycin selection [ 12 ]. Plasmid DNA is isolated using a modified cetyltriethylammoniumbromide (CTAB) extraction method [ 13 ]. Plasmid DNA is dissolved in water and stored at −20 °C. phlegethon\u0027s cuirassWebAug 24, 2024 · The appearance of weeds, resistant to EPSP synthase (EPSPS) inhibitors, acetolactate synthase (ALS), synthetic auxins, and photosystem II (PSII) inhibitors, have caused farming difficulties. Therefore, we have taken a genetic approach to herbicide resistance as a developing method in these GM crops. t strap shoes supplierWebexpression cassette encodes a phosphinothricin acetyltransferase gene from Streptomyces . viridochromogenes . that is codon-optimized for expression in corn. Method for conferring t strap shoe sockWebIn this study, we identified a tau-like glutathione transferase family gene from soybean named GmGSTU23. ... GmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance gene (phosphinothricin N-acetyltransferase, PAT) ... phleger kelley pictures to drawWebThe gene encodes phosphinothricin acetyltransferase, which N -acetylates phosphinothricin using acetyl-CoA. Sign in to download full-size image Figure 6.1. Overview of biosynthetic pathways of a number of phosphonate and phosphinate natural products. The first two steps of phosphonate biosynthesis (boxed) are conserved for most known … phlegethon tuchWebApr 11, 2024 · DP23211 corn also contains the gene that encodes for the phosphomannose isomerase protein, which is used as a selectable marker. ... using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate … phlegethon river underworldWebFeb 1, 2014 · The genes encoding for bialaphos resistance (phosphinothricin acetyltransferase), originally cloned from bialaphos producing streptomycetes [ 3, 56, 59, 63 ], have been used as selection markers in plant biology and engineering [ 11 ]. t strap shoes manufacturers