Cholesterylation of smoothened

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August undefined, 2024

WebSep 26, 2024 · So far, two proteins, namely Hedgehog and Smoothened, have been characterized to undergo cholesterylation. It is unknown whether other proteins are …

Cholesterol access in cellular membranes controls Hedgehog

WebHedgehog (Hh) is a morphogen that binds to its receptor Patched 1 and activates Smoothened (SMO), thereby governing embryonic development and postnatal tissue … WebOct 1, 2024 · The G-protein coupled receptor-like protein Smoothened (SMO) is one of the major members in Hh pathway. Covalent modification of cholesterol on the 95th … byu vs liberty football tickets

Jie LUO Wuhan University, Wuhan WHU Research profile

pCMV-FLAG-SMO was generated by inserting human Smo cDNA into pcDNA3, followed by inserting a 3× FLAG epitope-coding DNA after the signal peptide of SMO. The sequence after 1–192 amino acids (a.a.) was truncated to generate pCMV-FLAG-CRD. pCMV-GCaMP6s-SMO-mCherry-FLAG was generated as … See more The following antibodies were used in this study: mouse mAb anti-FLAG, clone M2 (F3165, Sigma), mouse mAb anti-acetyl Tubulin, clone 6-11B-1 (T6793, Sigma), mouse mAb anti-SMO, clone E5 (Sc-166685, Santa … See more HEK-293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) plus 10% fetal bovine … See more Cholesterol, thapsigargin, CuSO4and sodium ascorbate were from Sigma. Methyl-β-cyclodextrin and hydroxypropyl-β-cyclodextrin were from CTD. Inc. SAG, dantrolene sodium and GANT61 were from … See more Molecular docking studies were carried out using Molecular Operating Environment (MOE 2015.10, Chemical Computing Group, Canada) … See more WebCholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate. Article. Feb 2024; CELL RES; Ao Hu; Jing-Zan Zhang; Jie Wang; Chen-Chen Li; WebMar 1, 2024 · Smoothened (SMO), a class Frizzled G protein-coupled receptor (class F GPCR), transduces the Hedgehog signal across the cell membrane. ... Cholesterylation … byu vs liberty predictions

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Revealing the secret behind Smo cholesterylation - PMC

WebCholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate. Authors - Ao Hu. KO Validated; View Article. human: FC,IF: J Immunother Cancer. PD1 blockade enhances K+ channel activity, Ca2+ signaling, and migratory ability in cytotoxic T lymphocytes of patients with head and neck cancer. WebJan 1, 2024 · Cholesterol modification (or cholesterylation) is a rare but important posttranslational lipid modification of proteins. So far, two proteins, namely Hedgehog and Smoothened, have been ... cloudflare dns proxied openvpnWebMar 1, 2024 · Smoothened (SMO), a class Frizzled G protein-coupled receptor (class F GPCR), transduces the Hedgehog signal across the cell membrane. ... Cholesterylation of Smoothened is a calcium-accelerated ... byu vs liberty prediction

"WebFeb 1, 2024 · Request PDF Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate Hedgehog (Hh) is a … " - Cholesterylation of smoothened

Cholesterylation of smoothened

Revealing the secret behind Smo cholesterylation Request PDF

WebRegulation of Smoothened trafficking and abundance in Hedgehog signaling. Frontiers in Cell and Developmental Biology 10:847844. doi: 10.3389/fcell.2024.847844. Yuhong Han and Jin Jiang. 2024. Revealing the secret behind Smoothened Cholesterylation. Cell Research 32(4):327-328. doi: 10.1038/s41422-022-00636-8. WebOct 1, 2024 · The Hh signal reception system consists of a twelve-span transmembrane protein Patched (Ptc) that binds directly to Hh and a GPCR family of seven-span transmembrane protein Smoothened (Smo) that transduces the Hh signal across the plasma membrane. Mammals have two Ptc proteins: Ptch1 and Ptch2, with Ptch1 as the …

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WebSo far, two proteins, namely Hedgehog and Smoothened, have been characterized to undergo cholesterylation. It is unknown whether other proteins are similarly modified. … WebJun 1, 2024 · The G protein-coupled receptor (GPCR)-like protein Smoothened (SMO) is the central signal transducer in the Hh pathway. Cholesterol binds and then covalently links to the D95 residue of...

WebCholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate Article Feb 2024 Hedgehog (Hh) is a morphogen that binds to its receptor... WebApr 6, 2024 · A major unsolved question is how Hh signaling regulates the activity of Smoothened (SMO). ... the results of our study suggest that Hh signaling transduces to …

WebDec 16, 2024 · SMO cholesterylation is a calcium-accelerated autoprocessing reaction, and STIM1-ORAI1-mediated store-operated calcium entry promotes cholesterylation … WebCholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate Citing article ... Ubiquitin specific peptidase 20 (USP20) is a member of the...

WebThis article is cited by 2950 publications. Zheng-Wang Qu, Hui Zhu, Rainer Streubel, Stefan Grimme. Organo-Group 2 Metal-Mediated Nucleophilic Alkylation of Benzene: Crucial Role of Strong Cation−π Interaction.

WebApr 1, 2015 · Cholesterylation is a post-translational attachment of sterol to proteins. This modification has been a characteristic of a single family of hedgehog proteins (Hh). byu vs liberty ticketsWebThe G protein-coupled receptor (GPCR)-like protein Smoothened (SMO) is the central signal transducer in the Hh pathway. Cholesterol binds and then covalently links to the … byu vs liberty scoreWebThe GPCR family protein Smoothened (Smo) transduces Hedgehog signal across the plasma membrane. In a recent paper published in Cell Research, Hu et al. discovered that Smoothened undergoes an auto-cholesterylation process facilitated by calcium and demonstrated that covalent cholesterol attachment to Smo cysteine-rich domain is … byu vs liberty football 2022WebFeb 1, 2024 · Covalent SMO modification by cholesterol in its cysteine-rich domain (CRD) is essential for SMO function. SMO cholesterylation is a calcium-accelerated … byu vs liberty liveWebCholesterol modification (or cholesterylation) is a rare but important posttranslational lipid modification of proteins. So far, two proteins, namely Hedgehog and Smoothened, have … byu vs liberty football predictionsWebSMO cholesterylation is a calcium-accelerated autoprocessing reaction, and STIM1-ORAI1-mediated store-operated calcium entry promotes cholesterylation and activation of endosome-localized SMO. However, it is unknown whether the Hh-PTCH1 interplay regulates the activity of the endoplasmic reticulum (ER)-localized SMO. byu vs liberty play by playWebJan 18, 2024 · SMO cholesterylation is essential for Hh signaling and embryonic development and we are interested in understanding the underlying mechanisms but are also curious to find out if there are additional proteins modified by cholesterol. What kind of approach do you bring to your work? byu vs nevada football